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Successful dissemination of plasmid-mediated extended-Spectrum β-lactamases in enterobacterales over humans to wild fauna

Abstract : Background: The emergence of multidrug-resistant bacteria remains poorly understood in the wild ecosystem and at the interface of habitats. Here, we explored the spread of Escherichia coli containing IncI1-ST3 plasmid encoding resistance gene cefotaximase-Munich-1 (blaCTX-M-1) in human-influenced habitats and wild fauna using a genomic approach. Methods. Multilocus sequence typing (MLST), single-nucleotide polymorphism comparison, synteny-based analysis and data mining approaches were used to analyse a dataset of genomes and circularised plasmids. Results. CTX-M-1 E. coli sequence types (STs) were preferentially associated with ecosystems. Few STs were shared by distinct habitats. IncI1-ST3-blaCTX-M-1 plasmids are disseminated among all E. coli phylogroups. The main divergences in plasmids were located in a shuffling zone including blaCTX-M-1 inserted in a conserved site. This insertion hot spot exhibited diverse positions and orientations in a zone-modulating conjugation, and the resulting synteny was associated with geographic and biological sources. Conclusions. The ecological success of IncI1-ST3-blaCTX-M-1 appears less linked to the spread of their bacterial recipients than to their ability to transfer in a broad spectrum of bacterial lineages. This feature is associated with the diversity of their shuffling conjugation region that contain blaCTX-M-1. These might be involved in the resistance to antimicrobials, but also in their spread.
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https://hal.inrae.fr/hal-03315769
Contributor : Christine Molé <>
Submitted on : Thursday, August 5, 2021 - 7:54:20 PM
Last modification on : Tuesday, September 7, 2021 - 3:44:38 PM

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Racha Beyrouthy, Carolina Sabença, Frédéric Robin, Patricia Poeta, Giberto Igrejas, et al.. Successful dissemination of plasmid-mediated extended-Spectrum β-lactamases in enterobacterales over humans to wild fauna. Microorganisms, MDPI, 2021, 9 (7), pp.1-12. ⟨10.3390/microorganisms9071471⟩. ⟨hal-03315769⟩

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