Cysteine Proteome Reveals Response to Endogenous Oxidative Stress in Bacillus cereus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2021

Cysteine Proteome Reveals Response to Endogenous Oxidative Stress in Bacillus cereus

Abstract

At the end of exponential growth, aerobic bacteria have to cope with the accumulation of endogenous reactive oxygen species (ROS). One of the main targets of these ROS is cysteine residues in proteins. This study uses liquid chromatography coupled to high-resolution tandem mass spectrometry to detect significant changes in protein abundance and thiol status for cysteine-containing proteins from Bacillus cereus during aerobic exponential growth. The proteomic profiles of cultures at early-, middle-, and late-exponential growth phases reveals that (i) enrichment in proteins dedicated to fighting ROS as growth progressed, (ii) a decrease in both overall proteome cysteine content and thiol proteome redox status, and (iii) changes to the reduced thiol status of some key proteins, such as the transition state transcriptional regulator AbrB. Taken together, our data indicate that growth under oxic conditions requires increased allocation of protein resources to attenuate the negative effects of ROS. Our data also provide a strong basis to understand the response mechanisms used by B. cereus to deal with endogenous oxidative stress.
Fichier principal
Vignette du fichier
2021 - Publi 34 - Hamitouche - IntJMolSci.pdf (1.84 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-03316028 , version 1 (06-08-2021)

Licence

Identifiers

Cite

Fella Hamitouche, J. Armengaud, Luc Dedieu, Catherine Duport. Cysteine Proteome Reveals Response to Endogenous Oxidative Stress in Bacillus cereus. International Journal of Molecular Sciences, 2021, 22 (14), pp.7550. ⟨10.3390/ijms22147550⟩. ⟨hal-03316028⟩
41 View
74 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More