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Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis

Abstract : Abstract Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes.
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https://hal.inrae.fr/hal-03318287
Contributor : Taline Elmayan <>
Submitted on : Monday, August 9, 2021 - 4:50:33 PM
Last modification on : Monday, September 13, 2021 - 3:00:02 PM

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Nicolas Butel, Agnès Yu, Ivan Le Masson, Filipe Borges, Taline Elmayan, et al.. Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis. Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.1-12. ⟨10.1038/s41467-021-22995-3⟩. ⟨hal-03318287⟩

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