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Evaluation of open search methods based on theoretical mass spectra comparison

Abstract : Abstract Background Mass spectrometry remains the privileged method to characterize proteins. Nevertheless, most of the spectra generated by an experiment remain unidentified after their analysis, mostly because of the modifications they carry. Open Modification Search (OMS) methods offer a promising answer to this problem. However, assessing the quality of OMS identifications remains a difficult task. Methods Aiming at better understanding the relationship between (1) similarity of pairs of spectra provided by OMS methods and (2) relevance of their corresponding peptide sequences, we used a dataset composed of theoretical spectra only, on which we applied two OMS strategies. We also introduced two appropriately defined measures for evaluating the above mentioned spectra/sequence relevance in this context: one is a color classification representing the level of difficulty to retrieve the proper sequence of the peptide that generated the identified spectrum ; the other, called LIPR, is the proportion of common masses, in a given Peptide Spectrum Match (PSM), that represent dissimilar sequences. These two measures were also considered in conjunction with the False Discovery Rate (FDR). Results According to our measures, the strategy that selects the best candidate by taking the mass difference between two spectra into account yields better quality results. Besides, although the FDR remains an interesting indicator in OMS methods (as shown by LIPR), it is questionable: indeed, our color classification shows that a non negligible proportion of relevant spectra/sequence interpretations corresponds to PSMs coming from the decoy database. Conclusions The three above mentioned measures allowed us to clearly determine which of the two studied OMS strategies outperformed the other, both in terms of number of identifications and of accuracy of these identifications. Even though quality evaluation of PSMs in OMS methods remains challenging, the study of theoretical spectra is a favorable framework for going further in this direction.
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Contributor : Olivier DUPRE Connect in order to contact the contributor
Submitted on : Tuesday, August 10, 2021 - 2:33:12 PM
Last modification on : Friday, August 5, 2022 - 2:54:51 PM


Distributed under a Creative Commons Attribution 4.0 International License

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Albane Lysiak, Guillaume Fertin, Géraldine Jean, Dominique Tessier. Evaluation of open search methods based on theoretical mass spectra comparison. BMC Bioinformatics, BioMed Central, 2021, 22 (2 (Special Issue)), pp.65. ⟨10.1186/s12859-021-03963-6⟩. ⟨hal-03318623⟩



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