The genome of the cereal pest Sitophilus oryzae : a transposable element haven - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Preprints, Working Papers, ... Year : 2021

The genome of the cereal pest Sitophilus oryzae : a transposable element haven

Carlos Vargas-Chavez
  • Function : Author
Clément Goubert
Louis Beranger
  • Function : Author
Caroline Blanc
  • Function : Author
Aymeric Bonnamour
  • Function : Author
Matthieu Boulesteix
Nelly Burlet
  • Function : Author
Patrick Callaerts
Théo Chancy
  • Function : Author
André da Silva Barbosa
  • Function : Author
Elisa Dell’aglio
Alex Di Genova
Mariana Galvão Ferrarini
Alexandra Gerber
  • Function : Author
Benjamin Gillet
Robert Hubley
  • Function : Author
Sandrine Hughes
Emmanuelle Jacquin-Joly
Justin Maire
Marina Marcet-Houben
  • Function : Author
Florent Masson
Camille Meslin
  • Function : Author
Nicolas Montagne
  • Function : Author
Andrés Moya
  • Function : Author
Ana Tereza Ribeiro de Vasconcelos
Gautier Richard
Jeb Rosen
Marie-France Sagot
  • Function : Author
Arian F.A. Smit
  • Function : Author
Jessica Storer
  • Function : Author
Aurélien Vigneron
  • Function : Author
Waël Zamoum
  • Function : Author
Cristina Vieira
Amparo Latorre
  • Function : Author

Abstract

Abstract Among beetles, the rice weevil Sitophilus oryzae is one of the most important pests causing extensive damage to cereal in fields and to stored grains. S. oryzae has an intracellular symbiotic relationship (endosymbiosis) with the Gram-negative bacterium Sodalis pierantonius and is a valuable model to decipher host-symbiont molecular interactions. We sequenced the Sitophilus oryzae genome using a combination of short and long reads to produce the best assembly for a Curculionidae species to date. We show that S. oryzae has undergone successive bursts of transposable element (TE) amplification, representing 72% of the genome. In addition, we show that many TE families are transcriptionally active, and changes in their expression are associated with insect endosymbiotic state. S. oryzae has undergone a high gene expansion rate, when compared to other beetles. Reconstruction of host-symbiont metabolic networks revealed that, despite its recent association with cereal weevils (30 Kyear), S. pierantonius relies on the host for several amino acids and nucleotides to survive and to produce vitamins and essential amino-acids required for insect development and cuticle biosynthesis. In addition to being an agricultural pest and a valuable endosymbiotic system, S. oryzae can be a remarkable model for studying TE evolution and regulation, along with the impact of TEs on eukaryotic genomes.

Dates and versions

hal-03320933 , version 1 (16-08-2021)

Identifiers

Cite

Nicolas Parisot, Carlos Vargas-Chavez, Clément Goubert, Patrice Baa-Puyoulet, Severine Balmand, et al.. The genome of the cereal pest Sitophilus oryzae : a transposable element haven. 2021. ⟨hal-03320933⟩
77 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More