The transposable element-rich genome of the cereal pest Sitophilus oryzae - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Pré-Publication, Document De Travail Année : 2021

The transposable element-rich genome of the cereal pest Sitophilus oryzae

Carlos Vargas-Chavez
  • Fonction : Auteur
Clément Goubert
Patrice Baa-Puyoulet
Séverine Balmand
Louis Beranger
  • Fonction : Auteur
Caroline Blanc
  • Fonction : Auteur
Aymeric Bonnamour
  • Fonction : Auteur
Nelly Burlet
Federica Calevro
Patrick Callaerts
Théo Chancy
  • Fonction : Auteur
Stefano Colella
André da Silva Barbosa
  • Fonction : Auteur
Elisa Dell’aglio
Alex Di Genova
Gérard Febvay
  • Fonction : Auteur
Toni Gabaldon
  • Fonction : Auteur
Mariana Galvão Ferrarini
Alexandra Gerber
Benjamin Gillet
Robert Hubley
Sandrine Hughes
Justin Maire
Marina Marcet-Houben
Florent Masson
Camille Meslin
Nicolas Montagne
  • Fonction : Auteur
Andrés Moya
  • Fonction : Auteur
Gautier Richard
Jeb Rosen
Marie-France Sagot
  • Fonction : Auteur
Arian F.A. Smit
Jessica Storer
Carole Vincent-Monegat
Agnès Vallier
Aurélien Vigneron
Anna Zaidman-Remy
Waël Zamoum
  • Fonction : Auteur
Rita Rebollo
Amparo Latorre
Abdelaziz Heddi
  • Fonction : Auteur
  • PersonId : 748780
  • IdHAL : aziz-heddi

Résumé

Abstract Background The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and to stored grains. S. oryzae has an intracellular symbiotic relationship (endosymbiosis) with the Gram-negative bacterium Sodalis pierantonius and is a valuable model to decipher host-symbiont molecular interactions. Results We sequenced the Sitophilus oryzae genome using a combination of short and long reads to produce the best assembly for a Curculionidae species to date. We show that S. oryzae has undergone successive bursts of transposable element (TE) amplification, representing 72% of the genome. In addition, we show that many TE families are transcriptionally active, and changes in their expression are associated with insect endosymbiotic state. S. oryzae has undergone a high gene expansion rate, when compared to other beetles. Reconstruction of host-symbiont metabolic networks revealed that, despite its recent association with cereal weevils (30 Kyear), S. pierantonius relies on the host for several amino acids and nucleotides to survive and to produce vitamins and essential amino-acids required for insect development and cuticle biosynthesis. Conclusions Here we present the genome of an agricultural pest beetle, which may act as a foundation for pest control. In addition, S. oryzae may be a useful model for endosymbiosis, and studying TE evolution and regulation, along with the impact of TEs on eukaryotic genomes.

Dates et versions

hal-04706472 , version 1 (23-09-2024)

Identifiants

Citer

Nicolas Parisot, Carlos Vargas-Chavez, Clément Goubert, Patrice Baa-Puyoulet, Séverine Balmand, et al.. The transposable element-rich genome of the cereal pest Sitophilus oryzae. 2024. ⟨hal-04706472⟩
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