Skip to Main content Skip to Navigation
Journal articles

Docking of acetyl-CoA carboxylase to the plastid envelope membrane attenuates fatty acid production in plants

Abstract : Abstract In plants, light-dependent activation of de novo fatty acid synthesis (FAS) is partially mediated by acetyl-CoA carboxylase (ACCase), the first committed step for this pathway. However, it is not fully understood how plants control light-dependent FAS regulation to meet the cellular demand for acyl chains. We report here the identification of a gene family encoding for three small plastidial proteins of the envelope membrane that interact with the α-carboxyltransferase (α-CT) subunit of ACCase and participate in an original mechanism restraining FAS in the light. Light enhances the interaction between carboxyltransferase interactors (CTIs) and α-CT, which in turn attenuates carbon flux into FAS. Knockouts for CTI exhibit higher rates of FAS and marked increase in absolute triacylglycerol levels in leaves, more than 4-fold higher than in wild-type plants. Furthermore, WRINKLED1, a master transcriptional regulator of FAS, positively regulates CTI1 expression by direct binding to its promoter. This study reveals that in addition to light-dependent activation, “envelope docking” of ACCase permits fine-tuning of fatty acid supply during the plant life cycle.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03327726
Contributor : Camille Serva <>
Submitted on : Friday, August 27, 2021 - 2:31:33 PM
Last modification on : Sunday, August 29, 2021 - 3:20:17 AM

File

2020_Ye_ncomm.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Yajin Ye, Krisztina Nikovics, Alexandra To, Loic Lepiniec, Eric Fedosejevs, et al.. Docking of acetyl-CoA carboxylase to the plastid envelope membrane attenuates fatty acid production in plants. Nature Communications, Nature Publishing Group, 2020, 11 (1), ⟨10.1038/s41467-020-20014-5⟩. ⟨hal-03327726⟩

Share

Metrics

Record views

24

Files downloads

9