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Genome Editing in Y. lipolytica Using TALENs

Abstract : TALENs (Transcription Activator-Like EndoNuclease) are molecular scissors designed to recognize and introduce a double-strand break at a specific genome locus. They represent tools of interest in the frame of genome edition. Upon cleavage, two different pathways lead to DNA repair: Non-homologous End Joining (NHEJ) repair, leading to efficient introduction of short insertion/deletion mutations which can disrupt translational reading frame and Homology Recombination (HR)-directed repair that occurs when exogenous DNA is supplied. Here we introduce how to use TALENs in the oleaginous yeast Yarrowia lipolytica by presenting a step-by-step method allowing to knock out or to introduce in vivo a point mutation in a gene of Yarrowia lipolytica. This chapter describes the material required, the transformation procedure, and the screening process.
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Submitted on : Thursday, September 2, 2021 - 2:31:48 PM
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Coraline Rigouin, Christian Croux, Gwendoline Dubois, Fayza Daboussi, Florence Bordes. Genome Editing in Y. lipolytica Using TALENs. Wheeldon, I.; Blenner, M. Yarrowia lipolytica, 2307, pp.25-39, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1414-3_2⟩. ⟨hal-03332065⟩



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