Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles (Review Article) Epigenomes Year : 2021

Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering

Dynamique de la Chromatine , développement des plantes, ingéniérie épigénomique


CRISPR-based epigenome editing uses dCas9 as a platform to recruit transcription or chromatin regulators at chosen loci. Despite recent and ongoing advances, the full potential of these approaches to studying chromatin functions in vivo remains challenging to exploit. In this review we discuss how recent progress in plants and animals provides new routes to investigate the function of chromatin regulators and address the complexity of associated regulations that are often interconnected. While efficient transcriptional engineering methodologies have been developed and can be used as tools to alter the chromatin state of a locus, examples of direct manipulation of chromatin regulators remain scarce in plants. These reports also reveal pitfalls and limitations of epigenome engineering approaches that are nevertheless informative as they are often associated with locus- and context-dependent features, which include DNA accessibility, initial chromatin and transcriptional state or cellular dynamics. Strategies implemented in different organisms to overcome and even take advantage of these limitations are highlighted, which will further improve our ability to establish the causality and hierarchy of chromatin dynamics on genome regulation.
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hal-03354212 , version 1 (26-11-2021)




Annick Dubois, François Roudier. Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering. Epigenomes, 2021, 5 (3), pp.17. ⟨10.3390/epigenomes5030017⟩. ⟨hal-03354212⟩
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