HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Positive Interactions Between Lactic Acid Bacteria Could Be Mediated by Peptides Containing Branched-Chain Amino Acids

Abstract : Lactic acid bacteria (LAB) are responsible for the sanitary, organoleptic, and health properties of most fermented products. Positive interactions between pairs of LAB strains, based on nitrogen dependencies, were previously demonstrated. In a chemically defined medium, using milk and lupin proteins as sole nitrogen source, two proteolytic strains were able to sustain the growth of non-proteolytic strains, but one did not. The objective of the present study was, thus, to determine which specific peptides were implicated in the positive interactions observed. Peptides produced and involved in the bacterial interactions were quantified using tandem mass spectrometry (LC-MS/MS). About 2,000 different oligopeptides ranging from 6 to more than 50 amino acids in length were identified during the time-course of the experiment. We performed a clustering approach to decipher the differences in peptide production during fermentation by the three proteolytic strains tested. We also performed sequence alignments on parental proteins and identified the cleavage site profiles of the three bacterial strains. Then, we characterized the peptides that were used by the non-proteolytic strains in monocultures. Hydrophobic and branched-chain amino acids within peptides were identified as essential in the interactions. Ultimately, better understanding how LAB can positively interact could be useful in multiple food-related fields, e.g ., production of fermented food products with enhanced functional properties, or fermentation of new food matrices.
Complete list of metadata

https://hal.inrae.fr/hal-03520425
Contributor : Anne Thierry Connect in order to contact the contributor
Submitted on : Tuesday, January 11, 2022 - 8:54:05 AM
Last modification on : Friday, May 20, 2022 - 9:04:58 AM
Long-term archiving on: : Tuesday, April 12, 2022 - 6:35:59 PM

File

Canon2022_peptidomics.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Fanny Canon, Valérie Briard-Bion, Julien Jardin, Anne Thierry, Valérie Gagnaire. Positive Interactions Between Lactic Acid Bacteria Could Be Mediated by Peptides Containing Branched-Chain Amino Acids. Frontiers in Microbiology, Frontiers Media, 2022, 12, pp.793136. ⟨10.3389/fmicb.2021.793136⟩. ⟨hal-03520425⟩

Share

Metrics

Record views

18

Files downloads

26