Biochemical and physical–chemical characterisation of leaf proteins extracted from Cichorium endivia leaves - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Food Chemistry Année : 2022

Biochemical and physical–chemical characterisation of leaf proteins extracted from Cichorium endivia leaves

Résumé

This study provides a detailed characterisation of a leaf protein concentrate (LPC) extracted from Cichorium endivia leaves using a pilot scale process. This concentrate contains 74.1% protein and is mainly composed of Ribulose-1,5-BISphosphate Carboxylase/Oxygenase (RuBisCO). We show that the experimentally determined extinction coefficient (around 5.0 cm−1 g−1 L depending on the pH) and refractive index increment (between 0.27 and 0.39 mL g−1) are higher than the predicted ones (about 1.6 cm−1 g−1 L and 0.19 mL g−1, respectively). In addition, the UV–visible absorption spectra show a maximum at 258 nm. These data suggest the presence of non-protein UV-absorbing species. Chromatographic separation of the concentrate components in denaturing conditions suggests that RuBisCO SC may be covalently bounded to few phenolic compounds. Besides, the solubility of LPC proteins is higher than 90% above pH 6. Such high solubility could make LPC a good candidate as a functional food ingredient.
Fichier principal
Vignette du fichier
S0308814622002151.pdf (2.5 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03557587 , version 1 (22-07-2024)

Licence

Identifiants

Citer

Maude Ducrocq, Marie-Hélène Morel, Marc Anton, Valérie Micard, Sylvain Guyot, et al.. Biochemical and physical–chemical characterisation of leaf proteins extracted from Cichorium endivia leaves. Food Chemistry, 2022, 381, pp.132254. ⟨10.1016/j.foodchem.2022.132254⟩. ⟨hal-03557587⟩
67 Consultations
7 Téléchargements

Altmetric

Partager

More