Generation of deletion lines in allohexaploid Bread Wheat - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Book Sections Year : 2022

Generation of deletion lines in allohexaploid Bread Wheat

Abstract

Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopolyploidy. One way to face this challenge is to decrease the size of the interval bearing the gene of interest both genetically and physically, in order to reduce significantly the number of potential candidate genes. In this chapter, we describe a technical approach to produce chromosome-specific deletion lines to locate precisely genes of interest onto wheat chromosomes, a step forward to their cloning.
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Dates and versions

hal-03656818 , version 1 (02-05-2022)

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Heïdi Serra, Radim Svačina, Jan Bartoš, Pierre Sourdille. Generation of deletion lines in allohexaploid Bread Wheat. Plant Gametogenesis, 2484, Springer US, pp.183-199, 2022, Methods in Molecular Biology, 978-1-0716-2253-7. ⟨10.1007/978-1-0716-2253-7_14⟩. ⟨hal-03656818⟩
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