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Altered epithelial barrier functions in the colon of patients with spina bifida

Abstract : Our objectives were to better characterize the colorectal function of patients with Spina Bifida (SB). Patients with SB and healthy volunteers (HVs) completed prospectively a standardized questionnaire, clinical evaluation, rectal barostat, colonoscopy with biopsies and faecal collection. The data from 36 adults with SB (age: 38.8 [34.1-47.2]) were compared with those of 16 HVs (age: 39.0 [31.0-46.5]). Compared to HVs, rectal compliance was lower in patients with SB (p = 0.01), whereas rectal tone was higher (p = 0.0015). Ex vivo paracellular permeability was increased in patients with SB (p = 0.0008) and inversely correlated with rectal compliance (r = − 0.563, p = 0.002). The expression of key tight junction proteins and inflammatory markers was comparable between SB and HVs, except for an increase in Claudin-1 immunoreactivity (p = 0.04) in SB compared to HVs. TGFβ1 and GDNF mRNAs were expressed at higher levels in patients with SB (p = 0.02 and p = 0.008). The levels of acetate, propionate and butyrate in faecal samples were reduced (p = 0.04, p = 0.01, and p = 0.02, respectively). Our findings provide evidence that anorectal and epithelial functions are altered in patients with SB. The alterations in these key functions might represent new therapeutic targets, in particular using microbiota-derived approaches. Clinical Trials: NCT02440984 and NCT03054415.
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Contributor : Emilie Bernard Connect in order to contact the contributor
Submitted on : Monday, May 9, 2022 - 1:51:53 PM
Last modification on : Friday, September 23, 2022 - 10:26:05 AM
Long-term archiving on: : Wednesday, August 10, 2022 - 7:24:50 PM


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Charlène Brochard, Guillaume Bouguen, Raphael Olivier, Tony Durand, Sébastien Henno, et al.. Altered epithelial barrier functions in the colon of patients with spina bifida. Scientific Reports, Nature Publishing Group, 2022, 12 (1), pp.7196. ⟨10.1038/s41598-022-11289-3⟩. ⟨hal-03662541⟩



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