A single substitution in Vacuolar protein sorting 4 is responsible for resistance to Watermelon mosaic virus in melon - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Journal of Experimental Botany Year : 2022

A single substitution in Vacuolar protein sorting 4 is responsible for resistance to Watermelon mosaic virus in melon

Abstract

In plants, introgression of genetic resistance is a proven strategy for developing new resistant lines. While host proteins involved in genome replication and cell to cell movement are widely studied, other cell mechanisms responsible for virus infection remain under investigated. Endosomal sorting complexes required for transport (ESCRT) play a key role in membrane trafficking in plants and are involved in the replication of several plant RNA viruses. In this work, we describe the role of the ESCRT protein CmVPS4 as a new susceptibility factor to the Potyvirus Watermelon mosaic virus (WMV) in melon. Using a worldwide collection of melons, we identified three different alleles carrying non-synonymous substitutions in CmVps4. Two of these alleles were shown to be associated with WMV resistance. Using a complementation approach, we demonstrated that resistance is due to a single non-synonymous substitution in the allele CmVps4P30R. This work opens up new avenues of research on a new family of host factors required for virus infection and new targets for resistance.
Fichier principal
Vignette du fichier
Agaoua et al 2022 JEXBOT version acceptée avec figures.pdf (1009.09 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Licence
Public Domain

Dates and versions

hal-03671982 , version 1 (05-09-2023)

Licence

Public Domain

Identifiers

Cite

Aimeric Agaoua, Vincent Rittener, Christelle Troadec, Cecile Desbiez, Abdelhafid Bendahmane, et al.. A single substitution in Vacuolar protein sorting 4 is responsible for resistance to Watermelon mosaic virus in melon. Journal of Experimental Botany, 2022, 73, pp.4008-4021. ⟨10.1093/jxb/erac135⟩. ⟨hal-03671982⟩
104 View
66 Download

Altmetric

Share

More