Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Virology Year : 2022

Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells

Olivier Schwartz

Abstract

Bats are natural reservoirs of numerous coronaviruses, including the potential ancestor of SARS-CoV-2. Knowledge concerning the interaction between coronaviruses and bat cells is sparse. We investigated the ability of primary cells from Rhinolophus and Myotis species, as well as of established and novel cell lines from Myotis myotis, Eptesicus serotinus, Tadarida brasiliensis, and Nyctalus noctula, to support SARS-CoV-2 replication. None of these cells were permissive to infection, not even the ones expressing detectable levels of angiotensin-converting enzyme 2 (ACE2), which serves as the viral receptor in many mammalian species. The resistance to infection was overcome by expression of human ACE2 (hACE2) in three cell lines, suggesting that the restriction to viral replication was due to a low expression of bat ACE2 (bACE2) or the absence of bACE2 binding in these cells. Infectious virions were produced but not released from hACE2-transduced M. myotis brain cells. E. serotinus brain cells and M. myotis nasal epithelial cells expressing hACE2 efficiently controlled viral replication, which correlated with a potent interferon response. Our data highlight the existence of species-specific and cell-specific molecular barriers to viral replication in bat cells. These novel chiropteran cellular models are valuable tools to investigate the evolutionary relationships between bats and coronaviruses.

Domains

Virology
Fichier principal
Vignette du fichier
2021.05.31.446374.full.pdf (2.76 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03746441 , version 1 (25-11-2021)
hal-03746441 , version 2 (23-01-2024)

Identifiers

Cite

Sophie-Marie Aicher, Felix Streicher, Maxime Chazal, Delphine Planas, Dongsheng Luo, et al.. Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells. Journal of Virology, 2022, 96 (14), ⟨10.1128/jvi.00608-22⟩. ⟨hal-03746441v2⟩
207 View
24 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More