A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Experimental Botany Year : 2005

A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation

Abstract

A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C-13-norisoprenoidic compound, and a C-14-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching veraison was observed in both cultivars. In parallel, the C-13-norisoprenoid level increased from veraison to maturity in both cultivars.

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hal-03763658 , version 1 (29-08-2022)

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Sandrine Mathieu, Nancy Terrier, Jérôme Procureur, Frédéric Bigey, Ziya Günata. A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation. Journal of Experimental Botany, 2005, 56 (420), pp.2721-2731. ⟨10.1093/jxb/eri265⟩. ⟨hal-03763658⟩
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