Development, Validation, and Use of 1H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Molecules Year : 2022

Development, Validation, and Use of 1H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid

Asma Bourafai-Aziez
Connectez-vous pour contacter l'auteur
Daniel Jacob
Emmanuel Cassin
  • Function : Author
Annick Moing

Abstract

Acerola (Malpighia emarginata D.C.) is an exotic fruit with high agro-industrial potential due to its high content of ascorbic acid (AA), phenolic compounds, and carotenoid pigments. Acerola fruit is processed into concentrated juice or powder to be incorporated into food supplements. The ascorbic acid content of concentrated juice or powders must be controlled and well assessed. Therefore, the development of optimal methods and procedures for the rapid and accurate determination of the ascorbic acid content in juice concentrate and juice powder remains of considerable commercial interest. NMR spectroscopy is currently a powerful spectroscopic tool for the qualitative and quantitative analysis of molecules of all types and sizes. Firstly, this article presents the NMR-based metabolomic profiling of acerola juice and concentrate powder to describe and compare their composition. Thirty-six metabolites were identified. The AA over choline ratio and the NMR metabolomic profiles could be used for authentication in the future. Secondly, a rapid (8 min), reliable, and non-destructive method for the quantification of ascorbic acid by 1D 1H-NMR spectroscopy was developed and validated. The LOD and LOQ were 0.05 and 0.15 mg/mL, respectively. These two approaches could be combined to better characterize ingredients derived from acerola and incorporated into food supplements.
Fichier principal
Vignette du fichier
2022-Bourafai-Aziez-Molecules.pdf (2.24 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03767989 , version 1 (02-09-2022)

Licence

Attribution

Identifiers

Cite

Asma Bourafai-Aziez, Daniel Jacob, Gwladys Charpentier, Emmanuel Cassin, Guillaume Rousselot, et al.. Development, Validation, and Use of 1H-NMR Spectroscopy for Evaluating the Quality of Acerola-Based Food Supplements and Quantifying Ascorbic Acid. Molecules, 2022, 27 (17), pp.5614. ⟨10.3390/molecules27175614⟩. ⟨hal-03767989⟩
50 View
98 Download

Altmetric

Share

Gmail Facebook X LinkedIn More