The phenotype of the gut region is more stably retained than developmental stage in piglet intestinal organoids - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Frontiers in Cell and Developmental Biology Année : 2022

The phenotype of the gut region is more stably retained than developmental stage in piglet intestinal organoids

Mikael Albin
  • Fonction : Auteur
  • PersonId : 1260203
Caroline Achard
  • Fonction : Auteur
Laura Soler-Vasco

Résumé

Intestinal organoids are innovative in vitro tools to study the digestive epithelium. The objective of this study was to generate jejunum and colon organoids from suckling and weaned piglets in order to determine the extent to which organoids retain a location-specific and a developmental stage-specific phenotype. Organoids were studied at three time points by gene expression profiling for comparison with the transcriptomic patterns observed in crypts in vivo . In addition, the gut microbiota and the metabolome were analyzed to characterize the luminal environment of epithelial cells at the origin of organoids. The location-specific expression of 60 genes differentially expressed between jejunum and colon crypts from suckling piglets was partially retained (48%) in the derived organoids at all time point. The regional expression of these genes was independent of luminal signals since the major differences in microbiota and metabolome observed in vivo between the jejunum and the colon were not reproduced in vitro . In contrast, the regional expression of other genes was erased in organoids. Moreover, the developmental stage-specific expression of 30 genes differentially expressed between the jejunum crypts of suckling and weaned piglets was not stably retained in the derived organoids. Differentiation of organoids was necessary to observe the regional expression of certain genes while it was not sufficient to reproduce developmental stage-specific expression patterns. In conclusion, piglet intestinal organoids retained a location-specific phenotype while the characteristics of developmental stage were erased in vitro . Reproducing more closely the luminal environment might help to increase the physiological relevance of intestinal organoids.
Fichier principal
Vignette du fichier
fcell-10-983031.pdf (3.2 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03788364 , version 1 (26-09-2022)

Licence

Paternité

Identifiants

Citer

Eloïse Mussard, Corinne Lencina, Lise Gallo, Céline Barilly, Maryse Poli, et al.. The phenotype of the gut region is more stably retained than developmental stage in piglet intestinal organoids. Frontiers in Cell and Developmental Biology, 2022, 10, 14 p. ⟨10.3389/fcell.2022.983031⟩. ⟨hal-03788364⟩
46 Consultations
58 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More