Impact of food restriction on the medio‐basal hypothalamus of intact ewes as revealed by a large‐scale transcriptomics study - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Neuroendocrinology Year : 2022

Impact of food restriction on the medio‐basal hypothalamus of intact ewes as revealed by a large‐scale transcriptomics study

Abstract

In mammals, the medio-basal hypothalamus (MBH) integrates photoperiodic and food-related cues to ensure timely phasing of physiological functions, including seasonal reproduction. The current human epidemics of obesity and associated reproductive disorders exemplifies the tight link between metabolism and reproduction. Yet, how food-related cues impact breeding at the level of the MBH remains unclear. In this respect, the sheep, which is a large diurnal mammal with a marked dual photoperiodic/metabolic control of seasonal breeding, is a relevant model. Here, we present a large-scale study in ewes (n = 120), which investigated the impact of food restriction (FRes) on the MBH transcriptome using unbiased RNAseq, followed by RT-qPCR. Few genes (~100) were impacted by FRes and the transcriptional impact was very modest (<2-fold increase or < 50% decrease for most genes). As anticipated, FRes increased expression of Npy/AgRP/LepR and decreased expression of Pomc/Cartpt, while Kiss1 expression was not impacted. Of particular interest, Eya3, Nmu and Dio2, genes involved in photoperiodic decoding within the MBH, were also affected by FRes. Finally, we also identified a handful of genes not known to be regulated by food-related cues (e.g., RNase6, HspA6, Arrdc2). In conclusion, our transcriptomics study provides insights into the impact of metabolism on the MBH in sheep, which may be relevant to human, and identifies possible molecular links between metabolism and (seasonal) reproduction.
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Dates and versions

hal-03790164 , version 1 (28-09-2022)

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Hugues Dardente, Didier Lomet, Alice Desmarchais, Ophélie Téteau, Olivier Lasserre, et al.. Impact of food restriction on the medio‐basal hypothalamus of intact ewes as revealed by a large‐scale transcriptomics study. Journal of Neuroendocrinology, 2022, pp.1-13. ⟨10.1111/jne.13198⟩. ⟨hal-03790164⟩
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