Rapid pathogen detection is an emerging issue in clinical, environmental, and food industry sectors. Biosensors can represent a solution to culture-based and molecular methods as they respond to sensitivity, specificity, and rapidity needs. Screen-printed electrodes have been used in association with nanoparticles to increase the signal and improve sensitivityreaching low numbers of the targets. Antibodies, DNA probes, and aptamers are mainly used to functionalize the working electrodes to ensure high specific pathogen detection detection by the use of voltammetry, impedance spectroscopy, amperometry, and conductivity. Electrochemical biosensors can be minia-turized to construct portable devices useful for in situ assays.