Evolution of fluoroquinolone-resistant Escherichia coli in the gut after ciprofloxacin treatment - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue International Journal of Medical Microbiology Année : 2022

Evolution of fluoroquinolone-resistant Escherichia coli in the gut after ciprofloxacin treatment

Résumé

Background: Three healthy volunteers carried similar quinolone-resistant E. coli (QREC) (pulsed field gel electrophoresis profiles) in their gut before and after 14 days ciprofloxacin treatment. Given the intensity of the selective pressure and the mutagenic properties of quinolones, we determined whether these strains had evolved at the phenotypic and/or genomic levels. Material and methods: Commensal QREC from before day-0 (D0), and a month after 14 days of ciprofloxacin (D42) were compared in 3 volunteers. Growth experiments were performed; acetate levels, mutation frequencies, quinolone MICs and antibiotic tolerance were measured at D0 and D42. Genomes were sequenced and single nucleotide polymorphisms (SNPs) between D0 and D42 were analyzed using DiscoSNP and breseq methods. Cytoplasmic proteins were extracted, HPLC performed and proteins identified using X!tandem software; abundances were measured by mass spectrometry using the Spectral Counting (SC) and eXtraction Ion Chromatograms (XIC) integration methods. Results: No difference was found in MICs, growth characteristics, acetate concentrations, mutation frequencies, tolerance profiles, phylogroups, O-and H-types, fimH alleles and sequence types between D0 and D42. No SNP variation was evidenced between D0 and D42 isolates for 2/3 subjects; 2 SNP variations were evidenced in one. At the protein level, very few significant protein abundance differences were identified between D0 and D42. Conclusion: No fitness, tolerance, metabolic or genomic evolution of commensal QREC was observed overtime, despite massive exposure to ciprofloxacin in the gut. The three strains behaved as if they had been unaffected by ciprofloxacin, suggesting that gut may act as a sanctuary where bacteria would be protected from the effect of antibiotics and survive without any detrimental effect of stress.
Fichier principal
Vignette du fichier
2022_de Lastours_IJMM.pdf (871.75 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-03813234 , version 1 (13-10-2022)

Licence

Paternité - Pas de modifications

Identifiants

Citer

Victoire de Lastours, Rani El Meouche, F. Chau, J. Beghain, Didier Chevret, et al.. Evolution of fluoroquinolone-resistant Escherichia coli in the gut after ciprofloxacin treatment. International Journal of Medical Microbiology, 2022, 312 (2), pp.1-6. ⟨10.1016/j.ijmm.2022.151548⟩. ⟨hal-03813234⟩
54 Consultations
16 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More