Eimeria tenella ROP kinase 2 induces p38 MAPK cellular pathway - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Poster De Conférence Année : 2022

Eimeria tenella ROP kinase 2 induces p38 MAPK cellular pathway

Résumé

Eimeria tenella is an obligate intracellular parasite that causes avian coccidiosis. As for other apicomplexan parasites, such as Toxoplasma gondii, cell invasion and intracellular development rely on apical organelles content discharge, named micronemes and rhoptries. Some ROP kinases (ROPK) are key virulence factors in T. gondii. To date, among the 28 ropk encoded by E. tenella, only two were confirmed to be expressed at the sporozoite stage by proteomic analysis. We have previously shown that EtROP1 is implicated in the inhibition of the host cell apoptosis by interacting with the cellular p53. Here, we functionally characterized the second ROP kinase expressed at the sporozoite stage in E. tenella. EtROP2 is an active kinase that phosphorylates cell substrates of approximately 50 kDa. Its overexpression leads to the shortening of the prepatent period and to the early development of first generation schizonts. Conduction of RNA sequencing analysis and RT-qPCR on the host cell, allowed us to identified the MAPK pathway and the transcription factor cFos to be upregulated by EtROP2. We also showed by immunofluorescence assay that the active kinase EtROP2 is implicated in the p38 MAPK pathway activation. We established here, that EtROP2 activates the p38 MAPK pathway through a direct or indirect phosphorylation, leading to the overexpression of the master transcription factor cFos known to be implicated in E. tenella development.

Domaines

Parasitologie
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Dates et versions

hal-03852796 , version 1 (15-11-2022)

Identifiants

  • HAL Id : hal-03852796 , version 1

Citer

Adeline Ribeiro E Silva, Mamadou Diallo, Alix Sausset, Thomas Robert, Stéphane Bach, et al.. Eimeria tenella ROP kinase 2 induces p38 MAPK cellular pathway. Journées d’Animation Scientifique du Département Santé Animale (JAS SA), Oct 2022, Anglet, France. . ⟨hal-03852796⟩
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