Deciphering sex-specific miRNAs as heat-recorders in zebrafish - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2022

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

(1) , (2) , (3) , (2) , (1) , (2) , (1)
1
2
3

Abstract

In the last decade, a plethora of microRNAs (miRNAs) has been reported in a wide variety of physiological processes, including reproduction, in many aquatic organisms. However, miRNAome alterations occurred by environmental cues due to water temperature increment have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, the animal model zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation, a treatment that results in male-skewed sex ratios in the adulthood. Once the fish reached adulthood, gonads were sequenced by high-throughput technologies and a total of 23 and 1 differentially expressed miRNAs in ovaries and testes, respectively, were identified two months after the heat treatment. Most of these heat-recorder miRNAs were involved in human sex-related cancer and about 400 predicted-target genes were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of the predicted target genes, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected heat-recorder miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.
Fichier principal
Vignette du fichier
2022_vanGelderen_ScientificRep.pdf (5.09 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-03886962 , version 1 (06-12-2022)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Tosca A van Gelderen, Jérôme Montfort, José Antonio Álvarez-Dios, Violette Thermes, Francesc Piferrer, et al.. Deciphering sex-specific miRNAs as heat-recorders in zebrafish. Scientific Reports, 2022, 12, pp.18722. ⟨10.1038/s41598-022-21864-3⟩. ⟨hal-03886962⟩

Collections

LPGP INRAE PHASE
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More