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Book Sections Year : 2023

Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis

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Abstract

Next-generation sequencing technologies have impressively unlocked capacities to depict the complexity of microbial communities. Microbial community structure is for now routinely monitored by sequencing of 16S rRNA gene, a phylogenetic marker almost conserved among bacteria and archaea. Nevertheless, amplicon sequencing, the most popular used approach, suffers from several biases impacting the picture of microbial communities. Here, we describe an innovative method based on gene capture by hybridization for the targeted enrichment of 16S rDNA biomarker from metagenomic samples. Coupled to near full-length 16S rDNA reconstruction, this approach enables an exhaustive and accurate description of microbial communities by enhancing taxonomic and phylogenetic resolutions. Furthermore, access of captured 16S flanking regions opens link between structure and function in microbial communities.
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Dates and versions

hal-03912878 , version 1 (26-12-2022)

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Sophie Comtet-Marre, Oshma Chakoory, Pierre Peyret. Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis. Microbial Environmental Genomics (MEG), 2605, Springer US, pp.187-208, 2023, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2871-3_10⟩. ⟨hal-03912878⟩
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