The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein - Archive ouverte HAL Access content directly
Journal Articles Journal of Hazardous Materials Year : 2023

The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein

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Alicia Vallet
  • Function : relator_co_first_author
Jacqueline Martin-Laffon
Adrien Favier
Titouan Bonnot
Jean Armengaud
Bernhard Brutscher
Stéphane Ravanel
Jacques Bourguignon
  • Function : Correspondent author
Claude Alban
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Abstract

Uranium (U) is a naturally-occurring radionuclide that is toxic to living organisms. Given that proteins are primary targets of U(VI), their identification is an essential step towards understanding the mechanisms of radionuclide toxicity, and possibly detoxification. Here, we implemented a chromatographic strategy including immobilized metal affinity chromatography to trap protein targets of uranyl in Arabidopsis thaliana. This procedure allowed the identification of 38 uranyl-binding proteins (UraBPs) from root and shoot extracts. Among them, UraBP25, previously identified as plasma membrane-associated cation-binding protein 1 (PCaP1), was further characterized as a protein interacting in vitro with U(VI) and other metals using spectroscopic and structural approaches, and in planta through analyses of the fate of U(VI) in Arabidopsis lines with altered PCaP1 gene expression. Our results showed that recombinant PCaP1 binds U(VI) in vitro with affinity in the nM range, as well as Cu(II) and Fe(III) in high proportions, and that Ca(II) competes with U(VI) for binding. U(VI) induces PCaP1 oligomerization through binding at the monomer interface, at both the N-terminal structured domain and the C-terminal flexible region. Finally, U(VI) translocation in Arabidopsis shoots was affected in pcap1 null-mutant, suggesting a role for this protein in ion trafficking in planta.
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hal-03924852 , version 1 (06-01-2023)

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Alicia Vallet, Jacqueline Martin-Laffon, Adrien Favier, Benoît Revel, Titouan Bonnot, et al.. The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein. Journal of Hazardous Materials, 2023, 446, pp.art. 130668. ⟨10.1016/j.jhazmat.2022.130668⟩. ⟨hal-03924852⟩
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