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Article Dans Une Revue Biology Année : 2023

Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

Résumé

Odorant-binding proteins are soluble proteins abundantly secreted in the nasal mucus of vertebrates. Although their physiological functions are not known, these proteins are suspected to have a carrier role in solubilizing and carrying hydrophobic odorant molecules to the olfactory receptors. Here, we describe the expression of functional mouse mOBP5. The protein produced using bacteria was purified and characterized. We investigated its binding properties using a fluorescent competitive assay and microcalorimetry. Molecular docking experiments revealed hydrophobic residues in the binding cavity potentially involved in the stabilization of the odorant, thus explaining its binding properties.
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Dates et versions

hal-03994361 , version 1 (17-02-2023)

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Paternité

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Lucie Moitrier, Christine Belloir, Maxence Lalis, Yanxia Hou, Jérémie Topin, et al.. Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus. Biology, 2023, 12 (1), pp.2. ⟨10.3390/biology12010002⟩. ⟨hal-03994361⟩
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