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Preprints, Working Papers, ... Year : 2023

A methyl-seq tool to capture genomic imprinted loci

Abstract

Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role of imprinted genes in mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for a systematic evaluation. Here, we optimized and compared to bisulfite-based standard a novel methyl-seq system to capture 165 candidate regions for genomic imprinting and ultimately detect parent-of-origin methylation, the main hallmark of imprinting.

Dates and versions

hal-04099373 , version 1 (16-05-2023)

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Jean-Noël Hubert, Nathalie Iannuccelli, Cédric Cabau, Eva Jacomet, Yvon Billon, et al.. A methyl-seq tool to capture genomic imprinted loci. 2023. ⟨hal-04099373⟩
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