Background Intussusception diagnosis and surgical management in axolotls (Ambystoma mexicanum) is poorly documented. Case Description A client-owned, 5-year-old, sexually intact, female axolotl was presented for hyporexia of 4-week duration associated with regurgitation after feedings. Clinical examination showed lethargy, weight loss, and firm tissue at coelomic palpation. Coelomic ultrasonography was consistent with an intestinal intussusception. An exploratory coeliotomy was performed, followed by an intestinal resection and anastomosis of a thickened portion of intestinal loop. Following surgical excision of the invaginated intestinal loops, anorexia was not resolved, and the axolotl died 4 days later. A necropsy revealed a serofibrinous coelomitis. Histopathology confirmed the presence of an obstructive mass in the resected portion of the intestines. Conclusion and Case Relevance This report describes an intussusception diagnosis and attempted treatment in an axolotl. Ultrasonography in axolotls with nonspecific gastrointestinal symptoms is recommended for evaluation of the coelomic organs.