The increasing demand for water has made the planned use of water an issue of major concern. In this context, the use of treated wastewater (TWW) for irrigation appears as an alternative for solving water availability problems. Through irrigation, the soil endogenous microbiome is exposed to exogenous organisms (including pathogens, antibiotic resistant bacteria) and chemicals (like antibiotics) that may interfere with their growth and life but could also play a role in the mitigation of the dissemination of antimicrobial resistance (AMR). Indeed, interactions between these endogenous and exogenous microbiomes may rule out the AMR dissemination. In order to explore this point, lab-scale microcosms were set up using 2 soils with a four-year history of irrigation with (i) tap water and (ii) TWW, implying thus a different AMR pressure. Inside glass flasks, 25 g of each soil was weighed and an equivalent of 100 L/m2 irrigation water was added. Four type of water were tested with various microbiome load: tap water, TWW, treated and filtered 0.2 µm wastewater, treated and filtered 1 kDa wastewater. TWW was also added on the soils that were sterilized before irrigation in order to reduce the diversity of the endogenous microbiome. These flasks were incubated and sequential samplings were performed at 0, 7, 14, 30, 45, 60, 75, 90 days after irrigation. Sequencing and quantitative PCR analysis on soil and water samples will serve to understand the respective role of soil and water microbiome on AMR dissemination. Other agronomic data were also collected to verify their influence in biotic interactions. These data can serve for future decisions, mainly if we discover that the reclaimed water has an important role in biotic pressure and resistance spread. In this case, considering the possibility of filtering the reclaimed water before use it for irrigation will be a feasible alternative.