Long-term in vivo vitamin D3 supplementation modulates bovine IL-1 and chemokine responses - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Scientific Reports Year : 2023

Long-term in vivo vitamin D3 supplementation modulates bovine IL-1 and chemokine responses

Abstract

Vitamin D deficiency at birth, followed by prolonged insufficiency in early life may predispose bovine calves to infection and disease. However, the effects of vitamin D levels on innate immunity are unclear due to the lack of long-term supplementation trials in vivo and reliable approaches for reproducibly assessing immune function. Here, a standardized whole blood immunophenotyping assay was used to compare innate immune responses to infection relevant ligands (LPS, Pam3CSK4 and R848) between Holstein–Friesian calves supplemented with vitamin D (n = 12) from birth until 7 months of age and control calves (n = 10) raised on an industry standard diet. Transcriptomic analysis in unstimulated whole blood cells revealed increased expression of type I interferons and chemokines in vitamin D supplemented calves, while IL-1 and inflammasome gene expression was decreased. In response to stimulation with the bacterial ligand LPS, supplemented calves had significantly increased expression of CASP1 , CX3CR1 , CAT , whereas STAT1 was decreased . Stimulation with the bacterial ligand Pam3CSK4 revealed increased expression of IL1A , IL1B and CAT genes; and decreased C5AR1 expression. In response to the viral ligand R848, STAT1 and S100A8 expression was significantly decreased. An increased IL-1 and inflammasome gene expression signature in vitamin D supplemented calves in response to LPS and Pam3CSK4 was also found, with ELISA confirming increased IL-1β protein production. In contrast, a decreased chemokine gene expression signature was found in response to R848 in supplemented animals, with decreased IL-8 protein expression exhibited in response to all PAMPs also found. These results demonstrated expression of several cytokine, chemokine and inflammasome genes were impacted by vitamin D supplementation in the first 7 months of life, with IL-8 expression particularly responsive to vitamin D. Overall, vitamin D supplementation induced differential innate immune responses of blood immune cells that could have important implications for disease susceptibility in cattle.
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hal-04171140 , version 1 (26-07-2023)

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Cian Reid, Susana Flores-Villalva, Aude Remot, Emer Kennedy, Cliona O’farrelly, et al.. Long-term in vivo vitamin D3 supplementation modulates bovine IL-1 and chemokine responses. Scientific Reports, 2023, 13 (1), pp.10846. ⟨10.1038/s41598-023-37427-z⟩. ⟨hal-04171140⟩
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