Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/C as9 ribonucleoprotein and URA3 ‐based marker recycling - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Molecular Plant Pathology Year : 2023

Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/C as9 ribonucleoprotein and URA3 ‐based marker recycling

Abstract

Phytopathogenic fungi cause significant crop losses annually, leading to serious economic losses (Fisher et al., 2012; Oerke, 2006). Among them. Colletotrichum spp. are in the top 10 most economically and scientifically important phytopathogenic fungi, due to their wide host range and hemibiotrophic lifestyle (Dean et al., 2012). Members of the genus Colletotrichum are responsible for anthracnose disease in thousands of plant species and undergo, in general, two distinct phases during the infection process, namely, the biotrophic and necrotrophic phases (Cannon et al., 2012; Münch et al., 2008; Perfect et al., 1999). Infection begins with the development of melanized appressoria from fungal spores, which generate a high turgor pressure to penetrate the host plant's cuticle and cell wall. In the subsequent biotrophic phase, the fungi extend their mycelia within the living host cells to obtain nutrients. Infection is completed
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Dates and versions

hal-04174238 , version 1 (07-07-2023)
hal-04174238 , version 2 (31-07-2023)

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Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean‐félix Dallery, et al.. Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/C as9 ribonucleoprotein and URA3 ‐based marker recycling. Molecular Plant Pathology, 2023, ⟨10.1111/mpp.13378⟩. ⟨hal-04174238v2⟩
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