Unravelling specificities in wine yeast interactions - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Poster De Conférence Année : 2022

Unravelling specificities in wine yeast interactions

Cleo Conacher
  • Fonction : Auteur
  • PersonId : 1274294
Thérèse Marlin
  • Fonction : Auteur
  • PersonId : 1155781
Florian Bauer
  • Fonction : Auteur
  • PersonId : 1013703
Virginie Galeote
Thibault Nidelet

Résumé

The growing interest in new wine fermentation strategies, such as co-inoculating S. cerevisiae (Sc) and non- Saccharomyces (NS) species at the beginning of fermentation, requires a better understanding of how yeasts interact. Even though there are more and more studies on the interactions between Sc and NS species, few data are available on interactions between NS species. Moreover, it is still unknown whether interactions are more determined at the species or strain level. Thus, this study aims at determining the relative importance of species and strains in the interactions between 5 wine yeast species: Hanseniaspora uvarum (Hu), Lachancea thermotolerans (Lt), Starmerella bacillaris (Sb), Torulaspora delbrueckii (Td) and Sc. For this, we tested all possible pair cocultures between 15 strains (3 strains for each species) and compared their growth to monocultures. We followed the growth in synthetic must for 24 hours with a plate reader. This way, we were able to identify four different types of growth compared to monocultures: cases where the coculture growth was close to the average growth of both monocultures, where the coculture growth was closer to one of the monocultures, as well as cases where the coculture was either above or under both monocultures. This finding would indicate that in most cases coculture behaviour does not result from the additive growth of monocultures. Besides, the type of interaction seemed to be mainly determined at the species level whereas the strain level was mainly modulating the interaction intensity. These results constitute a first step to a better understanding of the microbial dynamics occuring in wine fermentation. It would then be interesting to study how these interactions develop in more complex consortia involving higher order interactions.
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Dates et versions

hal-04177022 , version 1 (03-08-2023)

Identifiants

  • HAL Id : hal-04177022 , version 1

Citer

Eleonore Pourcelot, Cleo Conacher, Thérèse Marlin, Florian Bauer, Virginie Galeote, et al.. Unravelling specificities in wine yeast interactions. ISSY36 - 36thInternational Specialised Symposium on Yeasts, Jul 2022, Vancouver, Canada. . ⟨hal-04177022⟩
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