The Salmonella virulence protein PagN is a potential promoter of bacterial escape from the Salmonella-containing vacuole to host cytosol
Résumé
Salmonella Typhimurium is a facultative intracellular entero-pathogen that remains a risk to public health worldwide. The tools and strategies allowing it to invade and survive within host cells are numerous. Nowadays, it is the only pathogen known to invade host cells using either a trigger or a zipper mechanism, respectively depending on the Type 3 secretion system-1, mainly encoded on Salmonella pathogenicity island-I, and on the two invasins Rck (1) and PagN (2, 3). Intracellularly, Salmonella are contained in a Salmonella-containing vacuole (SCV), whose maturation results in an acidic environment which is poor in divalent cations. Nevertheless, in the last decades, several studies gave some evidences for a Salmonella escape from the early SCV, leading to cytosolic hyper replication of the pathogen (4). As PagN expression was previously shown to be dependent on acidic pH and low divalent cation concentration (2), we explored the potential role of PagN in the escape of the SCV.
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