Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers.
Carolina Doerrier
(1)
,
Pau Gama Perez
(2)
,
Béatrice Chabi
(3)
,
Mario Ost
(4)
,
Giovanna Distefano
(5)
,
Dominik Pesta
(6)
,
Norma Ramon Dahdah
(2)
,
Helene Lemieux
(7)
,
Claudia Holody
(7)
,
Rowan G. Carpenter
(7)
,
Kersti Tepp
(8)
,
Marju Puurand
(8)
,
Tuuli Käämbre
(8)
,
Herve Dubouchaud
(9)
,
Fabienne Cortade
(3)
,
Elisa Calabria
(10)
,
Marta Pinna Casado
(11)
,
Marisol Fernandez-Ortiz
(12)
,
Darío Acuña‐castroviejo
(12)
,
Josep A Villena
(13)
,
Sander Grefte
(14)
,
Jaap Keijer
(14)
,
Kristin O'Brien
(15)
,
Alice P Sowton
(15)
,
Andrew James Murray
(15)
,
Matthew D Campbell
(16)
,
David J Marcinek
(16)
,
Edgar Nollet
(17)
,
Rob C. Wuest
(17)
,
Sudarshan Dayanidhi
(18, 19)
,
Stine Dam Soendergaard
(20, 21)
,
Karoline Maise Chroeis
(21, 20)
,
Bret H Goodpaster
(22)
,
Alba Gonzalez-Franquesa
(23)
,
Paul M. Coen
(22)
,
Hans Christian Steen‐larsen
(20, 21)
,
Erich Gnaiger
(1)
,
Pablo M Garcia-Roves
(2)
1
Oroboros Instruments
2 IDIBELL - Institut d'Investigació Biomèdica de Bellvitge = Bellvitge Biomedical Research Institute
3 DMEM - Dynamique Musculaire et Métabolisme
4 University Hospital Leipzig = Universitätsklinikum Leipzig
5 Florida Hospital
6 DLR Institute of Aerospace Medicine
7 University of Alberta
8 NICPB | KBFI - National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut [Estonie]
9 LBFA - Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée
10 Department of Neurological and Movement Sciences
11 Dept Molecular Cellular Pathology Therapy, Inst Biomedicina de Valencia
12 UGR - Universidad de Granada = University of Granada
13 Department of Psychiatric Genetics Unit, Vall d’Hebron Research Institute (VHIR), Barcelona, Catalonia, Spain
14 Human and Animal Physiology [Wageningen, The Netherlands]
15 Dept. of Physiology, Development and Neurosciences
16 Department of Radiology, University of Washington
17 Amsterdam Movement Sciences – Musculoskeletal Health Program, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands
18 RIC - Rehabilitation Institute of Chicago
19 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
20 Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark
21 Center for Healthy Aging [Copenhagen]
22 Translational Research Institute for Metabolism and Diabetes and the Burnham Institute
23 CBMR - Novo Nordisk Foundation Center for Basic Metabolic Research
2 IDIBELL - Institut d'Investigació Biomèdica de Bellvitge = Bellvitge Biomedical Research Institute
3 DMEM - Dynamique Musculaire et Métabolisme
4 University Hospital Leipzig = Universitätsklinikum Leipzig
5 Florida Hospital
6 DLR Institute of Aerospace Medicine
7 University of Alberta
8 NICPB | KBFI - National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut [Estonie]
9 LBFA - Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée
10 Department of Neurological and Movement Sciences
11 Dept Molecular Cellular Pathology Therapy, Inst Biomedicina de Valencia
12 UGR - Universidad de Granada = University of Granada
13 Department of Psychiatric Genetics Unit, Vall d’Hebron Research Institute (VHIR), Barcelona, Catalonia, Spain
14 Human and Animal Physiology [Wageningen, The Netherlands]
15 Dept. of Physiology, Development and Neurosciences
16 Department of Radiology, University of Washington
17 Amsterdam Movement Sciences – Musculoskeletal Health Program, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands
18 RIC - Rehabilitation Institute of Chicago
19 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
20 Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark
21 Center for Healthy Aging [Copenhagen]
22 Translational Research Institute for Metabolism and Diabetes and the Burnham Institute
23 CBMR - Novo Nordisk Foundation Center for Basic Metabolic Research
Béatrice Chabi
- Fonction : Auteur
- PersonId : 736660
- IdHAL : beatrice-chabi
- ORCID : 0000-0002-5604-5668
- IdRef : 077823672
Herve Dubouchaud
- Fonction : Auteur
- PersonId : 1160887
- IdHAL : hdubouchaud
- ORCID : 0000-0003-1268-6110
- IdRef : 176346856
Darío Acuña‐castroviejo
- Fonction : Auteur
- PersonId : 1367966
- ORCID : 0000-0002-9680-1560
Andrew James Murray
- Fonction : Auteur
- PersonId : 893086
Pablo M Garcia-Roves
- Fonction : Auteur
- PersonId : 1077906
Résumé
The exponential increase of scientific publications in the mitochondrial field shows the growing interest in mitochondria. However, the lack of methodological consistency in many published projects on mitochondrial respiratory function complicates a quantitative inter/intra-laboratory comparison of datasets. This deficiency manifests the need to improve the quality in science[1]. In this context, the MitoEAGLE COST Action is a powerful framework committed to evaluate and enhance the reproducibility in mitochondrial physiology as a basis to establish a novel mitochondrial database related to Evolution, Age, Gender, Lifestyle and Environment. Permeabilized muscle fibers are widely used to evaluate mitochondrial function in health and disease[2]. Therefore, our main goals are to: 1) compare protocols used in different research laboratories, 2) analyze factors which contribute to experimental variability, 3) define optimal experimental conditions in muscle studies, 4) elaborate guidelines for evaluating mitochondrial function in muscle tissue, 5) establish reference values on mitochondrial respiration, particularly as a test of the skills in preparing high-quality permeabilized muscle fibers, and 6) generate a database. To achieve our aims, two unique studies are currently in progress: 1) 17 international research groups performing independently experiments on respiration in permeabilized fibers of mouse soleus muscle, following the same experimental procedure[3]; 2) a blinded international study measuring simultaneously in the same laboratory respiration of permeabilized human skeletal fibers by high-resolution respirometry and assessing the effect of different experimental conditions[4]. Our results contribute to face the reproducibility crisis and provide the basis for establishing the first database on mitochondrial respiratory parameters in muscle tissues.