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Article Dans Une Revue The Plant cell Année : 2023

MYB24 orchestrates terpene and flavonol metabolism as light responses to anthocyanin depletion in variegated grape berries

Chen Zhang (1) , Zhanwu Dai (2) , Thilia Ferrier (3) , Luis Orduña (1) , Antonio Santiago (1) , Arnau Peris (1) , Darren Wong (4) , Christian Kappel (5) , Stefania Savoi (6) , Rodrigo Loyola (7) , Alessandra Amato (8) , Bartosz Kozak (9) , Miaomiao Li (10) , Akun Liang (11) , David Carrasco (12) , Carlos Meyer-Regueiro (7) , Carmen Espinoza (13) , Ghislaine Hilbert‐masson (3) , Rosa Figueroa-Balderas (14) , Dario Cantu (14) , Rosa Arroyo-Garcia (12) , Patricio Arce-Johnson (13) , Patricia Claudel (15) , Daniel Errandonea (11) , Manuel Rodríguez-Concepción (16) , Eric Duchêne (15) , Shao-Shan Carol Huang (10) , Simone Diego Castellarin (9) , Giovanni Battista Tornielli (8) , Francois Barrieu (3) , José Tomás Matus (11)
1 i2sysbio - Institute for Integrative Systems Biology [Valencia]
2 PSI - Key Laboratory of Plant-Soil Interactions - College of Resources and Environmental Sciences
3 UMR EGFV - Ecophysiologie et Génomique Fonctionnelle de la Vigne
4 ANU - Australian National University
5 University of Potsdam = Universität Potsdam
6 UNITO - Università degli studi di Torino = University of Turin
7 UC - Pontificia Universidad Católica de Chile
8 UNIVR - Università degli studi di Verona = University of Verona
9 UBC - University of British Columbia
10 NYU - New York University [New York]
11 UV - Universitat de València
12 UPM - Universidad Politécnica de Madrid
13 Universidad Autonoma de Chile
14 UC Davis - University of California [Davis]
15 SVQV - Santé de la vigne et qualité du vin
16 UPV - Universitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia
Chen Zhang
Institute for Integrative Systems Biology [Valencia]
Zhanwu Dai
Key Laboratory of Plant-Soil Interactions - College of Resources and Environmental Sciences
Thilia Ferrier
Ecophysiologie et Génomique Fonctionnelle de la Vigne
Luis Orduña
Institute for Integrative Systems Biology [Valencia]
Antonio Santiago
Institute for Integrative Systems Biology [Valencia]
Arnau Peris
Institute for Integrative Systems Biology [Valencia]
Darren Wong
Australian National University
Christian Kappel
University of Potsdam = Universität Potsdam
Stefania Savoi
Università degli studi di Torino = University of Turin
Rodrigo Loyola
Pontificia Universidad Católica de Chile
Alessandra Amato
Università degli studi di Verona = University of Verona
Bartosz Kozak
University of British Columbia
Miaomiao Li
New York University [New York]
Akun Liang
Universitat de València
David Carrasco
Universidad Politécnica de Madrid
Carlos Meyer-Regueiro
Pontificia Universidad Católica de Chile
Carmen Espinoza
Universidad Autonoma de Chile
Ghislaine Hilbert‐masson
Ecophysiologie et Génomique Fonctionnelle de la Vigne
Rosa Figueroa-Balderas
University of California [Davis]
Dario Cantu
University of California [Davis]
Rosa Arroyo-Garcia
Universidad Politécnica de Madrid
Patricio Arce-Johnson
Universidad Autonoma de Chile
Patricia Claudel
Santé de la vigne et qualité du vin
CV
Daniel Errandonea
Universitat de València
Manuel Rodríguez-Concepción
Universitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia
Eric Duchêne
Santé de la vigne et qualité du vin
Shao-Shan Carol Huang
New York University [New York]
Simone Diego Castellarin
University of British Columbia
Giovanni Battista Tornielli
Università degli studi di Verona = University of Verona
Francois Barrieu
Ecophysiologie et Génomique Fonctionnelle de la Vigne
José Tomás Matus
Universitat de València

Résumé

Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections of grape (Vitis vinifera cv. 'Bequignol') variegated berries and found that accumulation of products from branches of the phenylpropanoid and isoprenoid pathways showed an opposite tendency. Light-responsive flavonol and monoterpene levels increased in anthocyanin-depleted areas in correlation with increasing MYB24 expression. Cistrome analysis suggested that MYB24 binds to the promoters of 22 terpene synthase (TPS) genes, as well as 32 photosynthesis/light-related genes, including carotenoid pathway members, the flavonol regulator HY5 HOMOLOGUE (HYH), and other radiation response genes. Indeed, TPS35, TPS09, the carotenoid isomerase gene CRTISO2, and HYH were activated in the presence of MYB24 and MYC2. We suggest that MYB24 modulates ultraviolet and high-intensity visible light stress responses that include terpene and flavonol synthesis and potentially affects carotenoids. The MYB24 regulatory network is developmentally triggered after the onset of berry ripening, while the absence of anthocyanin sunscreens accelerates its activation, likely in a dose-dependent manner due to increased radiation exposure. Anthocyanins and flavonols in variegated berry skins act as effective sunscreens but for different wavelength ranges. The expression patterns of stress marker genes in red and white sections of 'Bequignol' berries strongly suggest that MYB24 promotes light stress amelioration but only partly succeeds during late ripening. MYB24 controls metabolic responses in skin sections of variegated grape berries lacking anthocyanin to cope with high-intensity and UV light stress, promoting terpene and flavonol accumulation.

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Sciences du Vivant [q-bio]

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https://hal.inrae.fr/hal-04288783

Soumis le : jeudi 16 novembre 2023-11:00:53

Dernière modification le : vendredi 12 avril 2024-18:32:06

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hal-04288783 , version 1 (16-11-2023)

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Chen Zhang, Zhanwu Dai, Thilia Ferrier, Luis Orduña, Antonio Santiago, et al.. MYB24 orchestrates terpene and flavonol metabolism as light responses to anthocyanin depletion in variegated grape berries. The Plant cell, In press, ⟨10.1093/plcell/koad228⟩. ⟨hal-04288783⟩

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