Differential titration of Plasmin and Plasminogen in milk using sandwich ELISA with monoclonal antibodies - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Dairy Research Year : 1997

Differential titration of Plasmin and Plasminogen in milk using sandwich ELISA with monoclonal antibodies

Abstract

A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly specific to plasminogen; the selectivity of this group was confirmed by immunoblots and BIA-core analysis. Cross-reactivity against horse, human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% were strictly bovine specific.
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Dates and versions

hal-04301377 , version 1 (23-11-2023)

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  • HAL Id : hal-04301377 , version 1

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Didier Dupont, Jeanne Grosclaude, Christelle Bailly, Jean-Claude Collin. Differential titration of Plasmin and Plasminogen in milk using sandwich ELISA with monoclonal antibodies. Journal of Dairy Research, 1997, 64. ⟨hal-04301377⟩
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