Monoclonal Antibodies against Bovine β-Casein: Production and Epitope Characterization - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Food and Agricultural Immunology Year : 2010

Monoclonal Antibodies against Bovine β-Casein: Production and Epitope Characterization

Abstract

Twenty one murine monoclonal antibodies (MAbs) were produced against bovine -casein (-CN). Eight epitopic groups of MAbs recognising six discrete determinants (4-8, 14-24, 33-48, 49-91, 177-183, 184-209) were defined from ELISA reactivity with peptides and related homologues from 3 species (ovine, human and equine). Thirteen distinct epitopes were discriminated by cross-examination of the MAbs reactivity in BIAcore. Determinants 14-24, 49-91 and 184-209 were found to encompass at lest 4, 2 and 4 epitopes respectively. No MAb was bovine specific, but one at least discriminated ovine/caprine casein from bovine. Terminal sequences 1-28 and 177-209 included ~80% of the discriminated epitopes, and the most conserved ones. This panel of MAbs can provide specific probes for -CN proteolysis fragments.
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Dates and versions

hal-04301391 , version 1 (23-11-2023)

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D. Senocq, Didier Dupont, O. Rolet-Répécaud, D. Levieux. Monoclonal Antibodies against Bovine β-Casein: Production and Epitope Characterization. Food and Agricultural Immunology, 2010, 13 (4), pp.213-224. ⟨10.1080/09540100120094483⟩. ⟨hal-04301391⟩

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