Contrasting Cd accumulation of Arabidopsis halleri populations: a role for (1→4)-β-galactan in pectin - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Hazardous Materials Year : 2023

Contrasting Cd accumulation of Arabidopsis halleri populations: a role for (1→4)-β-galactan in pectin

Xinhui An
  • Function : Author
Jean-Chrisologue Totozafy
  • Function : Author
  • PersonId : 1314197
Alexis Peaucelle
Catherine Yvonne Jones
  • Function : Author
William G.T. Willats
  • Function : Author
Herman Höfte
Massimiliano Corso
  • Function : Author
Nathalie Verbruggen

Abstract

Cadmium (Cd) accumulation is highly variable among Arabidopsis halleri populations. To identify cell wall (CW) components that contribute to the contrasting Cd accumulation between PL22-H (Cd-hyperaccumulator) and I16-E (Cd-excluder), Cd absorption capacity of CW polysaccharides, CW mono-and poly-saccharides contents and CW glycan profiles were compared between these two populations. PL22-H pectin contained 3-fold higher Cd concentration than I16-E pectin in roots, and (1 -> 4)-beta-galactan pectic epitope showed the biggest difference between PL22-H and I16-E. CW-related differentially expressed genes (DEGs) between PL22-H and I16-E were identified and corresponding A. thaliana mutants were phenotyped for Cd tolerance and accumulation. A higher Cd translocation was observed in GALACTAN SYNTHASE1 A. thaliana knockout and overexpressor mutants, which both showed a lengthening of the RG-I sidechains after Cd treatment, contrary to the wild-type. Overall, our results support an indirect role for (1 -> 4)-beta-galactan in Cd translocation, possibly by a joint effect of regu-lating the length of RG-I sidechains, the pectin structure and interactions between polysaccharides in the CW. The characterization of other CW-related DEGs between I16-E and PL22-H selected allowed to identify a possible role in Zn translocation for BIIDXI and LEUNIG-HOMOLOG genes, which are both involved in pectin modification.

Dates and versions

hal-04302361 , version 1 (23-11-2023)

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Xinhui An, Jean-Chrisologue Totozafy, Alexis Peaucelle, Catherine Yvonne Jones, William G.T. Willats, et al.. Contrasting Cd accumulation of Arabidopsis halleri populations: a role for (1→4)-β-galactan in pectin. Journal of Hazardous Materials, 2023, 445, pp.130581. ⟨10.1016/j.jhazmat.2022.130581⟩. ⟨hal-04302361⟩
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