Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation
Résumé
Methods: To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours. Results: Myeloid cells, i.e. granulocytes and monocytic cells including classical CD14 pos and non-classical/intermediate CD16 pos cells, were the dominantly recruited subsets, with the latter upregulating the membrane expression of MHC class II and CD80/86 molecules. Whereas corticosteroids did not reduce the different cell subset recruitment, they potently dampened the MHC class II and CD80/86 expression on monocytic cells and not on alveolar macrophages. Besides, corticosteroids induced a temporary and partial anti-inflammatory gene profile depending on cytokines and monocyte/macrophage subsets. Discussion: This work documents the baseline effects of the standard-of-care corticosteroid treatment for early innate allo-responses. These insights will enable further optimization and improvement of lung transplantation outcomes.
Mots clés
lung transplantation pig model monocytes-macrophages corticosteroids ischemiareperfusion section: allo-immunity and transplantation
lung
transplantation
pig model
monocytes-macrophages
corticosteroids
ischemiareperfusion section: allo-immunity and transplantation
lung transplantation pig model monocytes-macrophages corticosteroids ischemiareperfusion section: allo-immunity and transplantation
Domaines
Immunologie| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
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