Effect of the dietary supplement PERMEAPROTECT+ TOLERANCE© on gut permeability in a human co-culture epithelial and immune cells model
Résumé
Background and objective: The leaky gut syndrome is characterized by an intestinal hyperpermeability
observed in multiple chronic disorders. Alterations of the gut barrier are associated
with translocation of bacterial components increasing inflammation, oxidative stress and eventually
dysfunctions of cellular interactions at the origin pathologies. Therapeutic and/or preventive
approaches have to focus on the identification of novel targets to improve gut
homeostasis. In this context, this study aims to identify the role of PERMEAPROTECT + TOLERANE
©, known as PERMEA, a food complement composed of a combination of factors
(including L-Glutamine) known to improve gut physiology.
Methods: We tested the effects of PERMEA or L-Glutamine alone (as reference) on gut permeability
(FITC dextran method, expression of tight junctions) and its inflammatory/oxidative consequences
(cytokines and redox assays, RT-qPCR) in a co-culture of human cells (peripheral blood
mononuclear cells and intestinal epithelial cells) challenged with TNFα.
Results: PERMEA prevented intestinal hyperpermeability induced by inflammation. This was
linked with its antioxidant and immunomodulatory properties showing a better efficacity than LGlutamine
alone on several parameters including permeability, global antioxidant charge and
production of cytokines.
Conclusion: PERMEA is more efficient to restore intestinal physiology, reinforcing the concept that
combination of food constituents could be used to prevent the development of numerous diseases.
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité
Licence : CC BY - Paternité