The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Antimicrobial Agents and Chemotherapy Year : 2024

The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method

Abstract

Phage therapy has (re)emerged as a serious possibility for combating multidrug-resistant bacterial infections, including those caused by vancomycin-resistant Enterococcus faecium strains. These opportunistic pathogens belong to a specific clonal complex 17, against which relatively few phages have been screened. We isolated a collection of 21 virulent phages growing on these vancomycin-resistant isolates. Each of these phages harbored a typical narrow plaquing host range, lysing at most 5 strains and covering together 10 strains of our panel of 14 clinical isolates. To enlarge the host spectrum of our phages, the Appelmans protocol was used. We mixed four out of our most complementary phages in a cocktail that we iteratively grew on eight naive strains from our panel, of which six were initially refractory to at least three of the combined phages. Fifteen successive passages permitted to significantly improve the lytic activity of the cocktail, from which phages with extended host ranges within the E. faecium species could be isolated. A single evolved phage able to kill up to 10 of the 14 initial E. faecium strains was obtained, and it barely infected nearby species. All evolved phages had acquired point mutations or a recombination event in the tail fiber genetic region, suggesting these genes might have driven phage evolution by contributing to their extended host spectra.
Fichier principal
Vignette du fichier
lossouarn-et-al-2024-the-virtue-of-training-extending-phage-host-spectra-against-vancomycin-resistant-enterococcus.pdf (9.48 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-04557632 , version 1 (24-05-2024)

Licence

Attribution

Identifiers

Cite

Julien Lossouarn, Elsa Beurrier, Astrid Bouteau, Elisabeth Moncaut, Maria Sir Silmane, et al.. The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method. Antimicrobial Agents and Chemotherapy, 2024, 68 (5), pp. e0143923. ⟨10.1128/aac.01439-23⟩. ⟨hal-04557632⟩
7 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More