The whole blood oxylipin response : a new tool to assess and understand the inflammatory response to various immune stimuli in humans - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2024

The whole blood oxylipin response : a new tool to assess and understand the inflammatory response to various immune stimuli in humans

Etienne Villain
Bruno Charbit
Munyaradzi Musvosvi
  • Function : Author
  • PersonId : 1031309
Thomas J Scriba
Lluis Quintana-Murci
Darragh Duffy
Cécile Gladine

Abstract

Oxylipins are major immunomodulating mediators, yet studies of inflammation focus mainly on cytokines. Here, using a standardized whole-blood stimulation system, we characterized the oxylipin-driven inflammatory responses to various stimuli and their relationships with cytokine responses. We performed a pilot study in 25 healthy individuals using 6 different stimuli: 2 bacterial stimuli (LPS and live BCG), 2 viral stimuli (vaccine-grade poly I:C and live H1N1 attenuated influenza), an enterotoxin superantigen and a Null control. All stimuli induced a strong production of oxylipins but most importantly, bacterial, viral, and T cell immune responses show distinct oxylipin signatures. Integration of the oxylipin and cytokine responses for each condition revealed new immune networks improving our understanding of inflammation regulation. Finally, the oxylipin responses and oxylipin-cytokine networks were compared in patients with active tuberculosis or with latent infection. This revealed different responses to BCG but not LPS stimulation highlighting new regulatory pathways for further investigations.
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Dates and versions

hal-04604527 , version 1 (07-06-2024)

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  • HAL Id : hal-04604527 , version 1

Cite

Etienne Villain, Aurélie Chanson, Malwina Mainka, Nadja Kampschulte, Pauline Le Faouder, et al.. The whole blood oxylipin response : a new tool to assess and understand the inflammatory response to various immune stimuli in humans. 5th Annual Meeting of EpiLipidNET, May 2024, Dresde, Germany. ⟨hal-04604527⟩
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