Chromatin dynamics associated with desiccation tolerance/sensitivity during seed germination - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Conference Poster Year : 2023

Chromatin dynamics associated with desiccation tolerance/sensitivity during seed germination

Abstract

Mature dry seeds have a particular mechanism that allows them to survive with loss of >90% of water content called desiccation tolerance (DT). The seed-DT is tightly regulated, being acquired during seed development and lost shortly after germination. The timing of loss of DT after germinated can be extended by exogenous stimuli e.g., a mild osmotic stress (re-induction of the DT to germinated seeds). The molecular processes involved in DT acquisition/loss and re-induction have been well studied at the transcriptomic level, which resulted in identification of DT-associated gene networks. However, our understanding of molecular mechanisms that switch-on/off the genetic networks of seed-DT remains fragmentary, especially from epigenetic aspects. Here, we identified DT-core genes (a cluster of genes specifically associated with DT re-induction) in root tissues of germinated Medicago by RNA-Seq, followed by analyses to determine the chromatin status of the loci of these DT-core genes. ATAC-Seq revealed that the chromatin state of genomic regions containing the DT-core genes is clearly linked to their expression levels and phenotypes; more opened chromatin with higher gene expressions in desiccation tolerant-tissue stimulated by a mild osmotic stress, conversely more closed chromatin with lower expressions in desiccation sensitive-tissues especially at a later stage of post-germination. Additionally, ChIP-Seq for histone repressive marks detected a prominent signal of H3K27me3 on the DT core-gene sequences at the later stage tissue with condensed chromatin, suggesting that silencing of DT in post-germination developmental programs will be mainly due to the H3K27me3 marks by the action of the PRC2 complex.
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Dates and versions

hal-04665293 , version 1 (31-07-2024)

Identifiers

  • HAL Id : hal-04665293 , version 1

Cite

Naoto Sano, Jaiana Malabarba, Zhijuan Chen, Sylvain Gaillard, David Windels, et al.. Chromatin dynamics associated with desiccation tolerance/sensitivity during seed germination. 14th ISSS Conference 2023, Jul 2023, Paris, France. ⟨hal-04665293⟩
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