Accurate image segmentation is essential for image-based estimation of vegetation canopy traits, as it minimizes background interference. However, existing segmentation models often lack the generalization ability to effectively tackle both ground-based and aerial images across a wide range of spatial resolutions. To address this limitation, a cross-spatial-resolution image segmentation model for rice crop was trained using the integration of in-situ and in silico multi-resolution images. We collected more than 3,000 RGB images (real set) covering 17 different resolutions reflecting diverse canopy structures, illumination conditions and background in rice fields, with vegetation pixels annotated manually. Using the previously developed Digital Plant Phenotyping Platform, we created a simulated dataset (sim set) including 10,000 RGB images with resolutions ranging from 0.5 to 3.5 mm/pixel, accompanied by corresponding mask labels. By employing a domain adaptation technique, the simulated images were further transformed into visually realistic images while preserving the original labels, creating a simulated-to-realistic dataset (sim2real set). Building upon a SegFormer deep learning model, we demonstrated that training with multi-resolution samples led to more generalized segmentation results than single-resolution training on the real dataset. Our exploration of various integration strategies revealed that a training set of 9,600 sim2real images combined with only 60 real images achieved the same segmentation accuracy as 2,400 real images (IoU = 0.819, F1 = 0.901). Moreover, combining 2,400 real images and 1,200 sim2real images resulted in the best performing model, effective against six challenging situations, such as specular reflections and shadows. Compared with models trained with single-resolution samples and an established model (i.e., VegANN), our model effectively improved the estimation of both green fraction and green area index across spatial resoultions. The strategy of bridging real and simulated data for cross-resolution deep learning model is expected to be applicable to other crops. The best trained model is available at https://github. com/PheniX-Lab/crossGSD-seg.