GENOMIC REGIONS ASSOCIATED WITH THE COMPOSITION OF THE RABBIT CAECAL MICROBIOTA
Régions génomiques associées à la composition du microbiote caecal du lapin
Résumé
The aim of our study was to identify genomic regions influencing the composition of the rabbit caecal microbiota, to better understand its genetic determinism. The ribosomal 16S DNA was extracted from the microbial community of the caecum and the ribosomal 16S gene was then sequenced in 525 rabbits originated either from a line selected on feed efficiency or a control non-selected line. After rarefaction and filtration of the sequences, 859 OTUs (operational taxonomic units) were identified. The rabbits were genotyped with the Affimetrix Axiom Rabbit 200K chip. After quality control 514 rabbits and 103,072 SNPs were retained for association study analyses. Eighty-one QTLs were identified for 69 OTUs abundance, with a total of 492 significant SNPs (p < 0.05 genome-wide, corrected by the Bonferroni method). Most QTLs were associated with the abundance of a single OTU. Eight QTLs were associated with 2 or 3 OTUs.