Communication Dans Un Congrès Année : 2024

Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinus

Livia Sofrankova
Jan Perner
  • Fonction : Auteur
  • PersonId : 1266482
Steeve Thany

Résumé

The secretion of salivary gland (SG) in ticks is effectively triggered by the muscarinic acetylcholine receptor (mAChR) agonist, the pilocarpine, known as a worldwide-preferred tool for obtaining copious saliva. Presently, all we know is, that injection or apical application of pilocarpine, into/on partially-fed tick females induces robust long-lasting salivary secretion, whereas it fails to induce salivation from isolated SG. Cutting neuronal connections between tick synganglion and SG, support the involvement of a neural mAChR in these processes. Based on these observations, it has been generally believed that tick synganglion neurons, mediates SG fluid secretion via unidentified non-cholinergic “secreto-motor nerves”, connecting neuronal cells with SG. Currently, the only known processes, associating specific tick neurons with SG are the neuropeptidergic axons innervating either type II or both type II/III saliva-producing acini. Here, based on agonistic/antagonistic assessments along mutant-based verifications, we revealed that Ixodes ricinus tick, possesses two pharmacologically distinct types (-A and -B) of mAChR. The synganglion was suggested to sense cholinomimetics via rich axonal arborizations at its surface, originating from two clusters of peptidergic neurosecretory cells, each expressing either mAChR-A or -B. Furthermore, both sets of neuropeptidergic SG-innervating cells also possess a cholinoceptive feature by expressing either of these two receptor types on their terminals in saliva producing type II and III acini. Following this model, the effects of in vivo-injected selective muscarinic agents, put forward that co-joint action of two different axonal mAChRs mediate a tandem cooperation of type II/III SG acini for saliva cocktail formation.
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Dates et versions

hal-04805880 , version 1 (26-11-2024)

Identifiants

  • HAL Id : hal-04805880 , version 1

Citer

Ladislav Šimo, Caina Ning, J. Valdés James, Mateos-Hernández Lourdes, Sabine Rakotobe, et al.. Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinus. Entomology 2024 in Phoenix, Arizona, Entomological Society Of America, Nov 2024, Phoenix, United States. ⟨hal-04805880⟩
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