Bovine tuberculosis (bTB), caused by Mycobacterium bovis (Mb), is a chronic disease in cattle and a neglected cause of zoonotic human tuberculosis. In developed countries bTB remains a major economic burden. In humans, neutrophils are involved in innate immune resistance to M. tuberculosis. Evidence of resistance to Mb infection was also reported in contact cows, but mechanisms are still unknown. Neutrophils are overlooked in bTB. We recently identified a new neutrophil subset in cattle and mouse that shares the morphology of conventional inflammatory neutrophils, but expresses major histocompatibility complex class II (MHC-II) molecules. Only this later subset is able to suppress T lymphocyte proliferation, hence is regulatory.