Changes in H3K4me3 and H3K27me3 landscapes during in vitro derivation of embryonic stem cells -a bovine study
Résumé
In bovine, stem cells have only recently been stably derived from the blastocyst. Here, we compare the transcriptome and epigenomic landscape (H3K4me3 and H3K27me3) of embryonic stem cells (ESCs)with that of their in vivo counterparts, the inner cell mass (ICM) cells. We show substantial remodelling of the histone modification landscapes between in vivo and in vitro cells, reflecting transcriptomic changes. We also describe conserved and divergent features at potentially bivalent genes (containing both H4K4me3 and H3K27me3 at the promoter) between ICM and bESCs. In conclusion, our study shows that in vitro derivation of stem cells leads to changes in both the transcriptome and the epigenome. These changes could be due to adaptation of the culture, but also to developmental drift during derivation.
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