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Hdr Année : 2021

Molecular mechanisms controlling the DNA double-strand breaks (DSBs) repair in plants

Rajeev Kumar

Résumé

My long-standing research interest lies in the mechanisms of genome stability and meiosis with the main focus on meiotic recombination. After briefly exploring the plant lipid biosynthesis field in my Ph.D., I was interested in the DNA repair field and over the years I focused my research on the homologous recombination during meiosis using the mouse, yeast, and the current model Arabidopsis thaliana. During my postdoctoral study in the laboratory of Bernard de Massy, Montpellier, I identified and performed the functional characterization of two mouse proteins (MEI4 & REC114) involved in DNA double-strand (DSB) break formation, which initiates meiotic recombination. My postdoctoral research at Curie Institute in the laboratory of Valérie Borde unraveled the molecular mechanism that determines the length of gene conversion tracts during the repair of meiotic DSBs in budding yeast. In December 2014 I joined the laboratory of Raphaël Mercier as a research scientist (Chargé de recherché) at INRAE, Versailles where I undertook to investigate the role of the evolutionarily conserved FIGL1 complex that is implicated in the DSB repair during homologous recombination. Following the reorganization of the meiosis and recombination group at Versailles in 2019, I lead the research projects on the DSB repair thematic within the MeioMe team. I am interested in understanding how DSB repair is controlled at the molecular level. How these repair events occur in the context of the spatial organization of meiotic chromosomes. What are the genetic factors involved in plant DSB repair? and how can we modify the activity of plant DSB repair factors to increase meiotic crossover (CO) frequency for plant breeding? Keeping this background in mind, my current and future projects aim to develop three following research lines: 1) Investigating the role of the FIGL1 complex in the regulation of DNA strand invasion during DSB repair. This project is carried out in collaboration with Pauline Dupaigne, IGR, Villejuif, Frédéric Baudat, IGH, Montpellier and Jean Baptiste Charbonnier, CEA, Saclay with the financial support from ANR. 2) Characterizing function of a novel BRCA2 putative homolog in Physcomitrium patens (moss) in collaboration with Fabien Nogué in our group at IJPB. 3) Identifying Hype-rec chemical molecules for increasing meiotic CO frequency in plants. This project is developed in collaboration with a computational chemist team at the University of Strasbourg and is funded by Rijk Zwaan, a plant breeding company. These studies currently employ three full-time working persons – one Master's student, one Ph.D. student, and a postdoc – under my supervision. Taken together, I am leading, mentoring, and developing research projects addressing fundamental and translational questions of DSB repair with extramural funding using the muti-model and interdisciplinary approaches.
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tel-04500950 , version 1 (12-03-2024)

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  • HAL Id : tel-04500950 , version 1

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Rajeev Kumar. Molecular mechanisms controlling the DNA double-strand breaks (DSBs) repair in plants. Life Sciences [q-bio]. Habilitation à diriger des recherches de l'Université Paris-Saclay, 2021. ⟨tel-04500950⟩
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