Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Vitis Year : 2015

Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera

Abstract

In eukaryotic cells, 14-3-3 proteins constitute a family of conserved regulatory proteins which bind to a large number of phosphorylated partners. Although a great body of evidences accumulated in herbaceous species indicates that 14-3-3s play key roles in regulation of plant growth, information about function in tree plants is still very scarce. In this paper, we report the characterization of two novel 14-3-3s from grapevine (Vitis vinifera 'Cabernet Sauvignon') named VV1_CS and VV2_CS. The VV1_CS and VV2_CS cDNA were expressed in Escherichia coli and the recombinant proteins were shown to be functional proteins using plasma membrane H+-ATPase as target. Transcript analysis during grape development or under elicitor-induced stress revealed differential expression of the two 14-3-3 isoforms.
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Dates and versions

hal-01204156 , version 1 (23-09-2015)

Identifiers

  • HAL Id : hal-01204156 , version 1
  • PRODINRA : 293856
  • WOS : 000351257400006

Cite

Giovanni Farace, S. Visconti, Chloé Marchive, P. Aducci, M. Marra, et al.. Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera. Vitis, 2015, 54 (1), pp.33-40. ⟨hal-01204156⟩
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